›› 2010, Vol. 41 ›› Issue (2): 197-200.doi: 10.3969/j.issn.0529.1356.2010.02.006

• 论著 • 上一篇    下一篇

创伤后应激障碍模型大鼠内侧前额皮质磷酸化细胞外信号调节激酶1/2及EM>c-fos/EM>的变化

王海涛; 韩芳 ;石玉秀*    

  1. 中国医科大学基础医学院组织学与胚胎学教研室,沈阳 110001
  • 收稿日期:2009-05-06 修回日期:2009-06-02 出版日期:2010-04-06
  • 通讯作者: 石玉秀

Changes of phosphorylated ERK 1/2 and EM>c-fos/EM> in the medial prefrontal cortex of posttraumatic stress disorder rats

  1. Department of Histology and Embryology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China
  • Received:2009-05-06 Revised:2009-06-02 Online:2010-04-06
  • Contact: SHI Yu-xiu

关键词: 创伤后应激障碍, 内侧前额皮质, 细胞外信号调节激酶, c-fos, 免疫组织化学, 免疫印迹法, 反转录-聚合酶链式反应, 大鼠

Abstract: Objective To observe the changes of phosphorylated extracellular signalregulated kinase 1/2 (pERK1/2) and EM>c-fos/EM> in the medial prefrontal cortex (mPFC) of post-traumatic stress disorder (PTSD) rats. Methods Male Wistar rats were randomly divided into control group and PTSD model group. The model group rats were exposed to the singleprolonged stress (SPS) to set up the rat PTSD model. The expression of pERK1/2 was detected using immunohistochemistry and Western blotting, and the expression of EM>c-fos/EM> mRNA was detected using reverse transcriptionpolymerase chain reaction (RT-PCR). Results The result of immunohistochemistry analysis showed that the number of pERK1/2-positive cells of control group and model group were 10.4±2.07 and 48.8±10.08 respectively (EM>P/EM><0.01), and integral optical density were 24.955±3.691 and 110.810±10.643 respectively (EM>P/EM><0.01). And Western blotting showed that relative expression quantities of pERK/βactin were 0.510±0.052 and 1.109±0.106

Key words: Post-traumatic stress disorder, Medial prefrontal cortex, Extracellular signalregulated kinase, EM>c-fos/EM>, Immunohistochemistry, Western blotting, RT-PCR, Rat 

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